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Index > Protein center > CCL2(Gene name) > Bovine
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  • CCL2 (Gene name),
  • C-C motif chemokine 2 (Protein name ),  CCL2_BOVIN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Gene name:
    CCL2(SCYA2);
    Protein name:
    C-C motif chemokine 2;
    Alternative:
    Monocyte chemotactic protein 1A(MCP-1;MCP-1A);Acidic seminal fluid protein;Small-inducible cytokine A2;
    Organism:
    Bovine (Bos taurus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Chemotactic factor that attracts monocytes, but not neutrophils.
    Subcellular Location:
    N/A
    Protein Attributes:
    Sequence length:
    99
    Sequence:
    50:
    MKVSAALLCL | LLTVAAFSTE | VLAQPDAINS | QVACCYTFNS | KKISMQRLMN | 
    99:
    YRRVTSSKCP | KEAVIFKTIL | GKELCADPKQ | KWVQDSINYL | NKKNQTPKP
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    Pfam:
    String:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Bovine C-C motif chemokine 2
    MSDS:
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    ELISA Kit for Bovine C-C motif chemokine 2
    Cat.:
    E0087h
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    Packing:
    96T
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    ELISA Kit for Bovine C-C motif chemokine 2
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    E0087m
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    96T
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    ELISA Kit for Bovine C-C motif chemokine 2
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    E0087b
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    96T
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    ELISA Kit for Bovine C-C motif chemokine 2
    Cat.:
    E0087r
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    96T
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    ELISA Kit for Bovine C-C motif chemokine 2
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    E0087p
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    MSDS:
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    96T
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    ELISA Kit for Bovine C-C motif chemokine 2
    Cat.:
    E0087d
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    MSDS:
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    96T
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    CLIA Kit for Bovine C-C motif chemokine 2
    Cat.:
    U0087p
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine C-C motif chemokine 2
    Cat.:
    U0087r
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    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
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    CLIA Kit for Bovine C-C motif chemokine 2
    Cat.:
    U0087d
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    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine C-C motif chemokine 2
    Cat.:
    U0087b
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    CLIA Kit for Bovine C-C motif chemokine 2
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    U0087m
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    CLIA Kit for Bovine C-C motif chemokine 2
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    U0087h
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    96T
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    CLIA Kit for Bovine C-C motif chemokine 2
    MSDS:
    Please sign in first.
    Polyclonal Antibody for Bovine C-C motif chemokine 2
    Polyclonal Antibody for Bovine C-C motif chemokine 2
    Cat.:
    P0087Rb-r
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine C-C motif chemokine 2
    Polyclonal Antibody for Bovine C-C motif chemokine 2
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    P0087Rb-m
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine C-C motif chemokine 2
    Polyclonal Antibody for Bovine C-C motif chemokine 2
    Polyclonal Antibody for Bovine C-C motif chemokine 2
    Cat.:
    P0087Rb-h
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    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Bovine C-C motif chemokine 2
    Monoclonal Antibody for Bovine C-C motif chemokine 2
    Monoclonal Antibody for Bovine C-C motif chemokine 2
    Monoclonal Antibody for Bovine C-C motif chemokine 2
    Monoclonal Antibody for Bovine C-C motif chemokine 2
    Monoclonal Antibody for Bovine C-C motif chemokine 2
    Monoclonal Antibody for Bovine C-C motif chemokine 2
    Protein for Bovine C-C motif chemokine 2
    Cat.:
    R0087b
    Price:
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    Packing:
    1mg
    Purity:
    >90%
    Protein for Bovine C-C motif chemokine 2
    Protein for Bovine C-C motif chemokine 2
    Cat.:
    R0087d
    Price:
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    Packing:
    1mg
    Purity:
    >90%
    Protein for Bovine C-C motif chemokine 2
    Cat.:
    R0087p
    Price:
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    Packing:
    1mg
    Purity:
    >90%
    Protein for Bovine C-C motif chemokine 2
    Cat.:
    R0087h
    Price:
    Please sign in first.
    Packing:
    1mg
    Purity:
    >90%
    Protein for Bovine C-C motif chemokine 2
    Protein for Bovine C-C motif chemokine 2

    R&D Technical Data

    s

    12% SDS-PAGE analysis

             12% SDS-PAGE analysis

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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.3%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.5%

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    Recovery

    Recovery was determined by spiking various levels of CCL2 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    97

    91-99

    Plasma

    96

    94-103

     

     

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCL2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-98%

    96-103%

    97-105%

    102-108%

    EDTA plasma(n=5)

    96-101%

    98-105%

    99-106%

    91-96%

    heparin plasma(n=5)

    90-103%

     

    91-104%

    94-105%

    95-102%

     

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    References
    1. 1.
      "Gene expression and cDNA cloning identified a major basic protein constituent of bovine seminal plasma as bovine monocyte-chemoattractant protein-1 (MCP-1)."
      Wempe F. , Henschen A. , Scheit K.H.
      DNA Cell Biol.10:671-679(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Seminal plasma.
    2. 2.
      "Characterization by cDNA cloning of the mRNA of a new growth factor from bovine seminal plasma: acidic seminal fluid protein."
      Wempe F. , Einspanier R. , Scheit K.H.
      Biochem. Biophys. Res. Commun.183:232-237(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Seminal plasma.
    3. 3.
      "Characterization of the bovine monocyte chemoattractant protein-1 gene."
      Wempe F. , Kuhlmann J.K. , Scheit K.H.
      Biochem. Biophys. Res. Commun.202:1272-1279(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    4. 4.
      "U.S. veterinary immune reagent network: expressed bovine gene sequences."
      U.S. Veterinary Immune Reagent Network , Hudgens T. , Tompkins D. , Baldwin C.L.
      Submitted (2007-11) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: Belted Galloway.
      tissue: Peripheral blood.
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